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Semidry Blotting

 

Electrophoretic separation of proteins normally takes place in gel-like substances. They are often not susceptible to ligands (e.g. antibodies, lectins,…) of high molecular weight.
Therefore a transportation from the gels to an inert membrane is useful.

 

In semidry blotting systems the gel is covered by filtering paper impregnated with buffer solution.

 

Electrophoretic separated bands are transferred to membranes where they are available for specific and general purpose reagents.


 

Semidry Blotter

 

 

More Details

 

 
  Details: Semidry Blotter Art. Nr. 24-200

 

Characteristics

 

High electrical conductivity by transfer between a platinum coated titanium electrode and a high-grade steel electrode.

Due to the homogeneous electrical field and a high electrical field strength the low interelectrode gap leads to a faster and more even blotting process. Extra cooling is not necessary.

 

An automatic pressure mechanism ensures a perfect contact leading to the best possible transfer. Gas bubbles appearing during the transfer are leaving the blotting sandwich lateral without any negative influence on the blotting process.



Use of discontinuous and continuous buffer systems possible.